2018 : The effects of microorganism on coffee pulp pretreatment as a source of biogas production
Dr. Ir. Sri Rachmania Juliastuti M.Eng
Prof. Dr. Ir. Tri Widjaja M.Eng.
Coffee pulp waste composition consist of cellulose, hemicellulose, lignin, pectin and caffeine, tannin, and polyphenol as inhibitor substance. The high cellulose compound in coffee pulp can be used for alternative raw materials in the manufacture of biogas. This study aims to define the composition of the mixture of microorganisms of Pseudomonas putida, Trichoderma harzianum, and Aspergillus niger that are best to use in coffee pulp pretreatment to degrade inhibitor substance. The best result of pretreatment will be applied to biogas production. The first step is to do a pretreatment of the coffee pulp with variable Pseudomonas putida : Trichoderma harzianum : Aspergillus niger with a ratio of 1:1:1, 1:2:1, 1:1:2, 1:2:2, 2:1:1, 2:1:2, 2:1:1 (v:v:v), then variables that are most excellent in degrading inhibitor substance are selected. The second step, is doing anaerobic fermentation for 20 days at mesophilic temperature (30Â …