2013 : Cytotoxic and antioxidant activities of marine sponge diversity at Pecaron Bay Pasir Putih Situbondo East Java, Indonesia

Ir. Sri Nurhatika M.P


Abstract

ObjectiveA study on the cytotoxic and antioxidant activities of Sponges collected from Pecaron Bay Pasir Putih Situbondo was conducted. This study aimed at screening the hydro-ethanolic extracts of 7 sponge species: Callyspongia sp, Acanthella sp and Xestospongia sp colleted at the Pecaron Situbondo, East Java, Indonesia for antiproliferative and antioxidant activities.MethodsAfter collection, the species were immediately immersed in ethanol and stored at low temperatures (−20 °C) until use. Cytotoxic assay was conducted using MTT methods for some cancerous cells, including T47D, Casky and HT-29 meanwhile antioxidant assay was conducted using DPPH method.Resultsthe extracts from species A. suberitoides has the highest toxicity compare to another species which valued level on tumor cell lines (HT-29, T47D and Casky). Antioxidant assay using DPPH method found that only Aaptos suberitoides …