2011 : Detection ovarian cycle of bekantan (Nasalis larvatus) based on the profile of fecal estradiol and progesterone

Dr.Ir. Ria Asih Aryani Soemitro M.Eng.

Abstract

Based on the IUCN, Proboscis monkey (Nasalis larvatus) is one of primate with status endangered species. To support this species conservation programs, it has been conducted the monitoring ovarian cycles using fecal samples. Samples which used in this study were approximately of 210 fecal samples derived from 3 adult females which were kept in Gembira Loka zoo, Yogyakarta, Indonesia. Fecal sampling were withdrawn in the morning between 8-9 am for about 2 months. Subsequently, feces which obtained inserted into the ziplock plastic and stored in the freezer at temperature-20oC until to do the next step such as lyophilization, pulverization and extraction using methanol. Sample then assayed using EIA (Enzyme Immunoassay) method. Length of Estrous cycle was determined by calculating duration of peak estradiol hormone until peak of estradiol in the next cycle.The results showed that fecal estradiol levels were estimated from 5494.00 to 24645.606 pg/g dried weight feces and progesterone were 72.60-390.40 ng/g dried weight feces. The average of reproductive cycle is 22.66 days with a range of 22-23 days. Progesterone hormone cycle is shorter than estradiol (21-22 days). Although no further investigation, but this is expected because of differences in time lag between estradiol and progesterone. From the three adult female Bekantan which used in the study, 2 animals were diagnosed as fertile