Edwin Setiawan : The lysidyl aminoacyl transfer RNA synthetase intron, a new marker for demosponge phylogeographics ? case study on Neopetrosia

Edwin Setiawan S.Si., M.Sc

Year

2015

Published in

Journal of the Marine Biological Association of the UK

Authors

External link

Type

Jurnal Internasional

Keywords

Porifera sponges Neopetrosia marker nuclear DNA intron lysidyl aminoacyl transfer RNA synthetase LTRS


Abstract

Suitable genetic markers for population studies in sponges are necessary to further our understanding of biodiversity and dispersal patterns, and contribute to conservation efforts. Due to the slow mitochondrial substitution rates in demosponges, nuclear introns are among the preferable markers for phylogeographic studies, but so far only the second intron of the ATP synthetase beta subunit-gene (ATPS b) has been successfully established. In the present study, we analyse the intron of the Lysidyl Aminoacyl Transfer RNA Synthetase (LTRS), another potential marker to study demosponge intraspecific relationships, on samples ofNeopetrosia chaliniformis from various locations in the Indo-Pacific and compare its variation with a mitochondrial marker (CO2). LTRS recovers several reciprocal monophyletic groups among the Indo-Pacific N. chaliniformis and provides a potential alternative to ATPSb.