Setiyo Gunawan : Isolation and purification of fatty acid steryl esters from soybean oil deodorizer distillate
Setiyo Gunawan S.T., Ph.D
Depending on the source, deodorizer distillates usually have significantly different characteristics, uses, and value. Soybean oil deodorizer distillate (SODD) has been suggested as an alternative to marine animals as a natural source of squalene and a good raw material for the production of fatty acid steryl esters (FASEs), tocopherols, free phytosterols and fatty acids. The purpose of this work was to isolate and purify natural FASEs from SODD by a suitable method without degradation of the FASEs. Modified Soxhlet extraction, modified silica gel column chromatography, and binary solvent extraction were employed sequentially in this study to obtain FASEs from SODD. FASEs with a purity of 86.74% and a total recovery of 85.32% could be obtained in the final product.