Siti Machmudah : Hot compressed water extraction of polysaccharides from Ganoderma lucidum using a semibatch reactor
Dr.Eng. Siti Machmudah , ST.,M.Eng.
ASIA-PACIFIC JOURNAL OF CHEMICAL ENGINEERING
Ganoderma lucidum reishi glucan polysaccharides hot compressed water
Ganoderma lucidum (G. lucidum) is a mushroom-forming white rot fungus. Although not edible, it is grown commercially for use as a medicinal supplement. In this study, G. lucidum was utilized for the extraction of polysaccharides
by hot compressed water at temperatures of 373–463K and a pressure of 4.0 MPa using a semibatch system—a most effective method because water at high temperature and pressure behaves as a reaction medium with remarkable properties.
Under these conditions, thermal softening of G. lucidum occurred, allowing the removal of the polysaccharides protecting other constituents in G. lucidum via hydrolysis. The main massed peaks of water-soluble products were distributed at around
688–2638m/z with a peak-to-peak mass difference of 162m/z, consistent with the repeating unit of the glucans. On the basis of these results, this process is proposed as applicable to the isolation of polysaccharides from other types of biomass and
may lead to an advanced plant biomass components extraction technology.