Raden Darmawan : Isolation and Evaluation of Mycobacterium gilvum sp. Capable of Degrading PAHs as a Potential Strain for Bioremediation
Raden Darmawan S.T., M.T.
The 5th GelK International Symposium
Isolation, Characterization, Mycobacterium gilvum sp., PAHs, Bioremediation
Polycyclic aromatic hydrocarbons (PAHs) are pervasive organic pollutants belonging to the most hazardous environmental pollutants due to their characteristics such as toxic, mutagenic, carcinogenic effects. High concentration of PAHs were detected in sediment from Tanoura Bay - Yatsushiro Sea, Japan with mean concentration 30,200 ng/g dry weight. Two highest of PAHs concentration detected in bivalve samples from the same site are fluoranthene and pyrene with concentration of 110 ng/g wet wt and 70 ng/g wet wt, respectively . The strain was successfully isolated from soil collected on Tanoura Bay, Japan and was identified to Mycobacterium gilvum sp. PAHs degradation ability was evaluated by shaking test tubes at 180 rpm in 30 oC. They were consisting of 2 mL of minimum medium (MM), inserted PAHs (pyrene and fluoranthene) with final concentration of 100 mg/L and the strain picked up from gellan gum plate previously. Solid-phase extraction (SPE) was chosen on the PAHs extraction method for the estimation of PAHs residual concentration. The tube samples were extracted by using C18 cartridge prior to be injected to the HPLC. Mycobacterium gilvum sp. could degrade pyrene for 16 days incubation period at 100 %. While, degradation of fluoranthene could be achieved at 99.45 % for 28 days incubation. The isolated strain might be applied as potential strain for the bioremediation on the coastal sites polluted with PAHs.