Triono Bagus Saputro : In Vitro Selection of Maize (Zea mays) through NaCl Stress and Evaluation its Tolerance by Using Random Amplified Polymorphisme DNA (RAPD)

Triono Bagus Saputro S.Si, M.Biotech

Year

2015

Published in

International conference on Biological Science 2015

External link

-

Type

Seminar Internasional

Keywords

callus, selection, NaCl, random amplified polymorphism DNA (RAPD)


Abstract

Maize (Zea mays) is one of graminae plants that widely spread for many purposes wheter in food industry, feed, or bioenergy. Those high utilization required an increment in production, but unfortunately the needs were not meet to the demands since conversion of agricultural area increase lately. Indonesia has 99.093 km of shoreline that recognize as marginal land where the salinity is high as well. This research try to obtain varian from one of indoneasian cultivars that were able to live over those area. Talango and Manding varieties were used as an explant for callus induction stage. The callus were exposed to medium that contain NaCl (0, 2500, 5000, dan 7500 ppm). Furthermore, in 7500 ppm, Manding variety shows a better performance compared to Talango variety with 170 mg in weight increment while talango variety has no weight increment. The surviving callus were evaluate its tolerant status using RAPD technique with 10 primers. Those tenth primers are OPA 02, OPA 10, OPA 13, OPB 07, OPC 02, OPD 08, OPI 01, OPK 20, OPU 19, OPU 20. There are seven primers showing polymorphism to compare initial line and selected line in Talango variety. While eight primers showing polymorphism in Manding.